Analyzing the Efficacy of MinION DNA Sequencer with E.coli DNA
Click the Poster to View Full Screen, Right click to save image
Grant: NSF
Pranav Illendula
CoPIs:
Andrea Gallagher
College:
The Dorothy and George Hennings College of Science, Mathematics, and Technology
Major:
Biotechnology/Molecular Biology
Faculty Research Advisor(s):
Rongsun Pu
Abstract:
The MinION instrument exhibits the next generation of DNA sequencing. It uses nanopores to scan the electrical charge of the individual DNA bases passing through. Furthermore, the technology allows for handheld portability and on-the-site usage. The MinION sequencer's accuracy in producing reliable results within expected use was investigated. The instrument utilizes a flow cell containing the nanopores, a computer with appropriate software for the sequencing, and various buffers and reagents to prep the DNA. The experiment went through lambda and E.Coli DNA sequencing for control and experiment, respectively. The data collected is then compared using the known DNA sequence of the two samples to compare its accuracy. Therefore, the control sample with lambda DNA had an average accuracy of 91.5 % and an average identity of 96.9%. Additionally, the experimental sample with E. coli DNA was 90.0% and 95.3%, respectively. The error rate confirms that the reduced accuracy is only viable for on-field usage, and not for research purposes.